The Thermo Scientific ToxInsight IVT Platform
For scientists wishing to profile samples for toxic effects and action mechanisms, the Thermo Scientific ToxInsight IVT Platform offers a complete solution to significantly enhance in vitro toxicology (IVT) assessment. Designed to simplify your workflow by multiplexing targets in each cell, The ToxInsight IVT Platform can provide a clearer picture of potential toxic liabilities.

Traditional methods of assessing toxicity in vitro measure single targets in homogeneous formats and have a record of low predictive power. The ToxInsight IVT Platform leverages the proven power of automated cellular imaging to measure multiple toxicity indicators in each cell of the exposed population. Combined cellular outputs are automatically compared to untreated populations producing a more sensitive, multi-faceted risk assessment tool for in vitro toxicity testing.

The ToxInsight IVT Platform offers intuitive software, ready-made reagent cartridges and easy-to-interpret data analysis tools. The detailed image cytometry data acquired by multi-channel fluorescent imaging of cells in vitro allows analysis on a cell-by-cell basis, providing immediate phenotypic results that cannot be obtained by traditional biochemical assays.

Capabilities

• Superior sensitivity and specificity via unique assay endpoints including cell morphology and intracellular detail.
• Visual confirmation of results – if desired – by examining cellular images.
• A range of Thermo Scientific ToxInsight Cartridges offer appropriate reagents and analysis protocols for each toxicity target, with no assay development required
• Fully automated data capture and analysis.
• Compatible with samples in SBS-compliant micro-well plates.
• Innovative analytics provide a simple-to-interpret report on toxicity risk of a sample

Advantages of the ToxInsight IVT Platform

• Traditional methods of assessing toxicity measure single targets and have a record of low- predictive power.
• The ToxInsight IVT Platform leverages the proven power of cellular imaging, which combines multiple toxicity targets in single cells to provide better indicators of compound toxicity.
• Deeper insight into the mechanism of toxicity through multiple assay endpoints, measured on a cell-by-cell basis.
• ToxInsight endpoints provide greater sensitivity and specificity compared to traditional methods.
• Multiple assay parameters; offer reduced sample usage and rapid workflow.

Reader & Software

• Complete automation of image acquisition and analysis processes. No 'tuning' required.
• Simple-to-use software fits your workflow and gives results in minutes.
• No image analysis experience required.
• Small, bench top footprint.
• Ultra-stable solid-state illumination source offers maintenance free usage and robust data.
• Compatible with plate handling robots.

Data Analysis & Reporting

• Integrated software provides customizable reports that assist evaluation of the toxicity of compounds.
• Dose response plotting and EC/IC50 computation.
• Drill-down to cell-level measurements or images as desired.
• Export of data to popular third party software.

The Thermo Scientific ToxInsight Organelle Health Cartridge

• The Thermo Scientific ToxInsight Organelle Heath Cartridge enables simultaneous measurement of six cell-health parameters: cell loss, nuclear morphology, DNA content, cell membrane permeability, mitochondrial membrane potential changes and cytochrome c localization and release from mitochondria.
• Validated for use in HEPG2 and HK-2 cells to allow assessment of liver and kidney toxicity.

The Thermo Scientific ToxInsight Proliferation & Cell Death Cartridge

• The Thermo Scientific ToxInsight Proliferation and Cell Death Cartridge simultaneously quantifies nuclear DNA content, BrdU incorporation and active caspase 3 and p53 proteins.
• The Cartridge allows simultaneous measurements of cell proliferation (cell number, DNA replication) and apoptosis in single cells.
• Validated for use in HEPG2 cells to allow assessment of liver toxicity.

The Thermo Scientific ToxInsight Endocrine Profiler Panel
The Thermo Scientific ToxInsight Endocrine Profiler Panel (EPP) offers a complete solution for consumer products companies needing to comply with regulatory Reproductive Toxicity testing requirements. Traditional methods of assessing endocrine disruption in vitro measure single targets in homogeneous formats and have a record of low-predictive power. The ToxInsight Endocrine Profiler Panel leverages our proven technologies for automated cellular imaging and analysis for assessing the risk of human exposure to endocrine-active chemicals in the environment and measures estrogen and androgen receptor activity in human cell lines.

Each cartridge includes all the cell lines, reagents, assay protocols, growth media, plastic ware, analysis, and data-reporting capabilities to profile compounds for endocrine activity with high-sensitivity and specificity. The ToxInsight EPP cartridges, coupled to the ToxInsight Consumer and Health Products Safety (CHPS) Platform, offer a high-throughput, quantitative pre-screen for primary toxicology assessment, as well as a selection of chemicals for follow-up animal studies, in a simple mix-and-read format that is easily standardized. This automated, bench-top platform is easy-to-use and provides physiologically relevant toxicity assays in vitro, reducing the reliance on late-stage histopathology or animal studies. The ToxInsight CHPS Platform not only profiles compounds for their toxicity risk, but also determines the mechanism of action of any toxicity detected.

The Thermo Scientific ToxInsight Developmental Neurotoxicity Panel
The Thermo Scientific ToxInsight Developmental Neurotoxicity Panel (DNTP) offers a complete solution for companies needing an in vitro pre-screen for evaluating large number of compounds for general toxicity and neurotoxicity characteristics through the measurement of multiple biomarkers in individual cells. Traditional methods of assessing developmental neurotoxicity (DNT) require expensive and time-consuming in vivo tests. The predictive, animal-free, automated imaging approach, allows physiologically relevant assays to be utilized earlier in the screening process, saving time, expense and animal usage.

The Thermo Scientific ToxInsight IVT Platform, coupled with the ToxInsight DNTP is the only proven method for analyzing the effect of industrial chemicals on neuronal morphological chemicals in food, consumer products and the environment. The widespread usage of these chemicals has led to fears of a link to the increased incidence of neurological disorders in children. The assay uses NS-1 cells, a subclonal line of rat pheochromocytoma (PC-12) cells, which differentiate forming neurites when stimulated with nerve growth factor (NGF). Automated image cytometry allows quantitative morphological measurements of the fluorescently stained NS-1 cells to determine potential effects on neurodevelopment.

The Thermo Scientific ToxInsight Genotoxicity Panel
Chromosome mutations of both structure and number are implicated in many human diseases. There is substantial evidence that chromosome mutations and related events in oncogenes and tumor suppressor genes of somatic cells are involved in the induction and/or progression of some types of cancer in human and experimental animals. Thus, the purpose of both the in vitro Thermo Scientific ToxInsight Micronucleus and Comet Genotoxicity Panel Assays are to detect agents that modify chromosome structure and segregation, and through automated imaging analysis, using less compound, time, and overall costs associated with in vivo testing.

The Thermo Scientific ToxInsight Micronucleus Genotoxicity Panel (MNGP) is a standard genotoxicity assay used to assess the potential for compounds to produce chromosomal aberrations. Manual scoring of the micronucleus assay is labor intensive, therefore automation of this task is desired. The ToxInsight MNGP allows for automated and rapid quantification of binucleated cells and micronuclei required for the assay. In the presence of a genotoxic agent (with or without metabolic activation), whole chromosomes or broken DNA may be left behind as micronuclei. The assay automatically analyzes each image set and applies the criteria for targeted cells and micronuclei consistently, ensuring a non-biased analysis. Addition of cytochalasin B improves the assay by allowing you to target only those cells that have successfully reached the binucleate phase, thus allowing distinction between cytotoxic and genotoxic agents. An optional third marker can be used to detect cells with compromised plasma membranes.

The Thermo Scientific ToxInsight Comet Genotoxicity Panel (CGP) is an automated tool for imaging, analyzing and reporting DNA damage by acute exposure to chemicals of radiation. The simple, fast, validated, imaging approach is used to measure Comet formation in vitro in a single channel assay. The easy to investigate DNA damage and chromosomal rearrangements combine comet analysis with fluorescent in situ hybridization (FISH) probes. With real time image analysis and data, you can report for instant access to results. Balancing speed and flexibility, The ToxInsight CGP captures more information from each well than any manual method.

The Thermo Scientific ToxInsight Drug Induced Liver Injury
For drug developers, finding liver toxicity late in the drug discovery process is not only frustrating, but also costly. The Thermo Scientific ToxInsight IVT Platform and ToxInsight Drug Induced Liver Injury (DILI) Assay provides more information on the mechanisms of liver toxicity earlier in the discovery process. The ToxInsight DILI Cartridge offers a new multi-parameter, cell-based assay to determine the risk of liver injury by chemicals being tested for new drugs or for consumer products safety assessment. Unlike traditional assays that measure single targets, The ToxInsight DILI Cartridge analyzes compound hepatotoxicity by simultaneous detection of multiplexed targets, including cell loss, nuclear DNA, reduced glutathione level, intracellular reactive oxygen species and mitochondrial membrane potential. The ToxInsight Solution unites imaging instrumentation, software and reagents in a simple, intuitive workflow to deliver a more effective and predictive toxicity filter for your discovery process.