NMR Spectroscopy Service

Because QA requirements vary, GLP/GMP work must be arranged in advance. Please contact Acorn NMR to discuss your GLP/GMP needs.

The most common service provided is basic one dimensional spectra of common nuclei (1H, 31P, 19F and 13C). However, all 1D and 2D experiments can be performed, and most NMR-active nuclei can be observed. A variable temperature controller allows experiments to be run at both high and low temperatures. The JEOL 400 is a state-of-the-art spectrometer, equipped with indirect detection capability and a Z-gradient which provides excellent solvent suppression and faster, higher quality 2D spectra using gradient selected experiments.

We recognize the time-sensitive nature of NMR analysis. In most cases, 1H spectra are run and data returned to the customer the day the samples are received. Other nuclei and more extensive analysis may require 1-2 days, but we will make every effort to accommodate urgent requests with advance notice.

Data can be emailed to the customer, for those who want the ability to examine, plot and archive data, prepare reports, etc. A ShareWare version of the NUTS NMR data processing software is available for viewing and processing the data. The customer can choose to receive processed or unprocessed data, depending on how much processing (s)he prefers to do. Alternatively, spectra can be faxed for immediate review, and original spectra returned via mail. Samples can be returned if requested (shipping charge applies).

Customers are encouraged to take full advantage of our expertise and the capabilities we offer. A brief description of experiments commonly performed is provided for consideration:

DEPT for assignment of 13C spectra
COSY to determine 1H-1H assignments
HMQC and HMBC to help in assigning 1H and 13C spectra and for complete structure analysis
NOESY for assigning 1H spectra and distinguishing between isomers

Our spectroscopists have extensive experience in application of NMR to chemical problems and can provide customized analysis ranging from acquiring reference spectra to detailed molecular structure determination. Special projects, such as method development for industrial process applications, can be arranged. We strive to provide analysis tailored to the needs of each customer and sample. This works best if the customer explains what type of information (s)he is looking for - for example, if quantitation of low level impurities is needed, the sample is run longer than for a crude reaction intermediate. The more we know, the better job we can do in customizing the experiment. A nondisclosure agreement can be executed if the customer desires, to facilitate discussion of chemical structures and problems.

A sample submission form is available, which can be printed, filled out and included when shipping samples. All sample submissions should be accompanied by this form.

There are no hidden costs, no surcharges for tubes, solvents (except unusual solvents), faxing and mailing spectra or returning or disposing of samples (other than return shipping fees). There is no minimum charge, and no extra charge for same-day service.

For typical samples, the analysis includes:
Preparation of the sample using our tubes and solvents
Data acquisition, processing and plotting of the spectrum with integrals, peak lists and expanded plots as needed
Spectral interpretation, if requested
Emailing the data, or FAXing and mailing spectra
Return or disposal of the sample, per customer request

Dos and Don'ts of sample packaging:
Samples should be placed into small vials, with the top secured with parafilm
Place the vials in a ziplock bag or equivalent, in case of leakage
Use sufficient packing material to guard against breakage
A sample submission form must be included, but please do not put it into the ziplock bag containing samples

Please * do not use * the cardboard overnight shipping envelopes designed for documents - we suspect these packages are processed on a conveyor belt using giant rollers, because glass sample vials are reduced to powder in the process.

Acorn NMR Inc., 7670 Las Positas Rd., Livermore, CA 94550. Tel: 925-456-1020; Fax: 925-456-1024.